Unlike woodland edges, flower strips do not act as a refuge for cabbage stem flea beetle aestivation.

BACKGROUND: Semi-natural habitats are generally considered to be beneficial to natural enemies of crop pests and pollinators. However, they could also be used by pests, such as the Cabbage Stem Flea Beetle (CSFB), Psylliodes chrysocephala, a major pest of winter oilseed rape, Brassica napus. Adults emerge from pupation in late spring and move to aestivation habitats. Published reports identify forest edges as the major shelter used, but flower strips may also constitute an alternative habitat. This study aimed to: (i) determine the role of perennial flower strips in CSFB aestivation in comparison with woodland edges; (ii) determine the influence of landscape composition on the abundance of aestivating CSFB and (iii) identify the characteristics of the local habitat associated with a high abundance of aestivating CSFB. RESULTS: CSFB emergence from aestivation was monitored with emergence traps from mid-August to mid-October 2021, at 14 sites in France. We found that CSFB preferred woodland edges and did not aestivate in flower strips. We found a negative effect of percentage woodland cover only for the smallest scale studied (250 m radius). We also found positive effects of the percentage of litter and mean tree circumference on the number of aestivating CSFB in woodland edges. CONCLUSION: The aestivation of CSFB is supported by woodland edges, but not by flower strips. This implies that the presence of flower strips near oilseed rape fields does not exacerbate the problems due to this pest. However, the crops in the vicinity of woodlands could be colonized earlier by this pest than more distant fields. © 2023 Society of Chemical Industry.

Initial assemblage characteristics determine the functional dynamics of flower-strip plant communities.

In agroecosystems, species-rich habitats, such as linear field margins and flower strips, are beneficial to the overall biodiversity and contribute to pest control. Their effects are thought to be mediated by plant species composition and diversity. However, the management of plant communities with targeted levels of functional diversity has been little investigated. In an open field landscape, we compared the effects of the sown species richness (9, 14, and 29 species) and functional diversity (high vs. low) of eight different seed mixtures, sown in flower strips, on the 4-year temporal dynamics of their functional diversity. There was a good agreement between the expected and realized species richness and functional diversity at the start of the experiment. All plant assemblages progressively lost species over time, but this decline was lower for assemblages sown with a high initial functional diversity, in which species evenness was maintained at higher levels. Species-rich assemblages had a higher degree of functional redundancy, and their functional diversity remained higher over time than less rich assemblages. A possible explanation for this is that functional redundancy would have enabled the compensation for the loss of species by functionally equivalent species. The realized functional diversity of the sown species also limited the establishment of spontaneous species, perhaps due to a higher degree of niche occupancy. This study provides useful insight into the creation of functionally diversified plant communities. A high level of initial species and functional diversity is required to guarantee a greater temporal persistence of the communities.

Exploring multitrophic interactions in oilseed rape fields reveals the prevailing role of Carabidae.

In cropped fields, birds are often at the highest position in the food chain, feeding on pest arthropods and their intermediate predators in a process known as intraguild predation. The net effects of bird predation on phytophagous insect populations (feeding on plants) are difficult to predict without comprehensively describing prey-predator communities and their complex interplay. We sampled bird and arthropod communities in 30 oilseed rape fields in the spring of 2019 and 2020 in France. To assess the top-down control of arthropods by birds, we used a vertebrate exclusion experiment. Using a taxonomic and functional trait-based approach, we determined the direct and indirect influences of birds on arthropod predators and phytophagous insect populations in arable crops. We observed a negative relationship between the abundance of Carabidae and phytophagous insects but not with the other predator group suggesting the key role of Carabidae on phytophagous insects in agroecosystem. We found no statistical evidence of intraguild predation from birds toward intermediate predators. Despite the lack of overall effect of predator functional diversity on their prey, we highlighted the negative relationship between the functional complementarity (through functional evenness) of Carabidae and the abundance of phytophagous insects. This result suggests that functional complementarity between Carabidae species could help to reduce phytophagous insect populations. We analyzed the effect of agricultural practices on these multitrophic interactions, showing that pesticide intensity only had detrimental effects on Carabidae abundance, while the frequency of tillage did not affect the studied communities. Complementary indices used to depict communities are helpful to better understand the mechanisms underlying trophic relationships.

The hump-shaped effect of plant functional diversity on the biological control of a multi-species pest community.

Plant taxonomic and functional diversity promotes interactions at higher trophic levels, but the contribution of functional diversity effects to multitrophic interactions and ecosystem functioning remains unclear. We investigated this relationship in a factorial field experiment comparing the effect of contrasting plant communities on parasitism rates in five herbivore species. We used a mechanistic trait-matching approach between plant and parasitoids to determine the amount of nectar available and accessible to parasitoids. This trait-matching approach best explained the rates of parasitism of each herbivorous species, confirming the predominant role of mass-ratio effects. We found evidence for an effect of functional diversity only in analyses considering the ability of plant communities to support the parasitism of all herbivores simultaneously. Multi-species parasitism was maximal at intermediate levels of functional diversity. Plant specific richness had a negligible influence relative to functional metrics. Plant communities providing large amounts of accessible nectar and with intermediate levels of functional diversity were found to be the most likely to enhance the conservation biological control of diverse crop herbivores.

Which Pitfall Traps and Sampling Effort to Choose to Evaluate Cropping System Effects on Spider and Carabid Assemblages?

In arable agroecosystems, arthropod communities often have a reduced abundance and diversity, which represents a challenge for sampling techniques needed to detect small differences among these simplified communities. We evaluated the suitability of pitfall traps for comparing the effects of cropping systems on arthropod communities. In a field experiment, we compared the effects of two pitfall trap diameters, the type of preserving fluid and the sampling effort on three metrics (activity density, taxonomic richness, and community weighted mean [CWM] of body size) for carabids and spiders. Trap size affected the observed composition of communities, with large traps yielding a higher proportion of spiders, and a higher richness and CWM body size for both taxa. The type of preserving fluid had a weaker effect. Simulations with various sampling efforts showed that only very different communities could be distinguished with less than 10 traps per field or less than 30 field replicates. Fewer traps were required to find differences between cropping systems for body size than for other metrics. Carabid activity density and body size, and spider genus richness, were the variables better distinguishing between cropping systems with the smallest sampling effort. A high sampling effort was required for comparing activity density and richness across cropping systems. Selection of the most appropriate trap design, metrics, and crops are the main factors for optimizing the trade-off between sampling effort and the ability to detect arthropod community responses to habitat management.

Limited genetic structure and demographic expansion of the Brassicogethes aeneus populations in France and in Europe.

BACKGROUND: The pollen beetle, Brassicogethes aeneus (Fabricius, 1775), is one of the most significant pests of oilseed rape. To shed light on past and current pollen beetle demography (dispersal, population size), 12 microsatellite markers were developed, and population genetic diversity and structure were analysed at different spatial scales in France and in Europe from 433 individuals collected in 18 winter oilseed rape fields. RESULTS: Genetic differentiation among the population samples was low but was significant between the Estonian sample and the rest of Europe. Isolation by distance was significant only at the European scale. Genetic variability was similar among the 18 population samples. Demographic inferences suggested a recent expansion of B. aeneus population size over Europe, possibly corresponding to an increase in oilseed rape crop area during past decades. CONCLUSION: Current population size and dispersal are not straightforward to estimate from the distribution of genetic variability in B. aeneus over Europe because of the complexity of the demographic history of this pest. Nevertheless, because gene flow was important enough to prevent strong genetic differentiation at large geographical scales, the management of pollen beetle populations should likely be thought of at a continental Europe level. © 2018 Society of Chemical Industry.

Characterization of the pollen beetle, Brassicogethes aeneus, dispersal from woodlands to winter oilseed rape fields.

Many crop pests rely on resources out of crop fields; understanding how they colonize the fields is an important factor to develop integrated pest management. In particular, the time of crop colonization and damage severity might be determined by pest movements between fields and non-crop areas. Notably, the pollen beetle, Brassicogethes aeneus, previously named Meligethes aeneus, one of the most important pests of winter oilseed rape, overwinters in woodlands. As a result, its abundance increases in oilseed rape fields near wooded areas. Here, we assessed the spatio-temporal patterns of the dispersal from woodlands to oilseed rape fields in diversified landscapes of a same region. We observed on four dates the abundance of pollen beetles in 24 fields spread in the Eure department, France. We modeled the abundance as a result of the dispersal from the neighboring woodlands. We compared the modalities of dispersal corresponding to different hypotheses on the dispersal origin, kernel shape and sources of variability. Within oilseed rape the distance to the edges of woodlands is not the main determinant of pollen beetle abundance. On the contrary, the variability of the abundance between fields is largely explained by the dispersal from neighboring woodlands but there is considerable variability between dates, sites and, to a lesser extent, between fields. The two dispersal kernels received similar support from the data and lead to similar conclusions. The mean dispersal distance is 1.2 km but seems to increase from a few hundred meters the first week to more than two kilometers the fourth, allowing the pollen beetles to reach more distant OSR fields. These results suggest that early varieties away from woodlands and late varieties close to the woodlands may limit attacks at the time when oilseed rape is the most sensitive.